banana dna extraction lab report conclusion

Use the wooden stirrer to start poking the cloudy stuff in the alcohol layer. The two strands are held together by hydrogen bonds between adenine and thymine and between guanine and cytosine. For instance, the detergent or soap helps to break down the cells outer membrane, and the salt helps to separate the DNA from other materials in the cell. This complementarity between adenine and cytosine nitrogenuos base pairs and between guanine and cytosine nitrogenuos base pase pairs, attracted to one another by hydrogen bonds, is critical to genetic function. 0000001999 00000 n Mashing the banana exposes a greater surface area from which to extract the DNA. % (60 oC) 2. When we added. 0000003809 00000 n Close the bag and very gently squeeze and move the saltwater and banana mush together. (B) Banana Extraction 1. (Remove the skin of the bananas and kiwi, we just want the insides!) The process involves a few general steps, including mashing, filtration, precipitation, and extraction. JFIF d d C You don't want the mixture to become too foamy. However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. During the DNA extraction, the soap pulls apart the fats (lipids) and proteins that make up the membranes surrounding the cell and nucleus. A probable reason for this The solution then sat for two to three minutes without a single disruption such as shaking the test tube. Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. An academic unit ofThe College of Liberal Arts and Sciences, You may need to edit author's name to meet the style formats, which are in most cases "Last name, First name. For more info, see, https://askabiologist.asu.edu/activities/banana-dna, Public Service and 7 0 obj In this study, we evaluate eight commonly used DNA extraction methods to determine their efficacy in PCR analysis across life stages and preservation . All living things including humans, plants (bananas!) 0000002160 00000 n Place the banana onto a plate. These instructions are in segments of DNA called genes. We do in fact share about 50% of our genes with plants including bananas. Bananas have 44.1% of genetic makeup in common with humans. Humans share 50% of our DNA with a banana.. &t #s, #"cre%#b$y sma$$' but we ca" see #t by extract#"g DN a"%, %#st#$$e% water #" the p$ast#c bag' sea$ the bag a"%, so$ut#o" s$ow$y !or about a m#"ute u"t#$ the shampoo, the empty cup. We use cookies to give you the best experience possible. /SM 0.02 The Essay on Human Genome Project Sequence Dna Information. Step 2: Put the banana into a bag. 9. However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. DNA extractions the surface of genetic engineering. 3 For what purposes is DNA extraction done give at least 3 purposes for which you may need to extract DNA? A.deletion B.duplication C.translocation D.transposition E.inversion 2 Which amino acid can sometimes be present at the P site without first. Seeing Banana Banana DNA Extraction Activity Ask A Biologist activity for classroom and home Table of Contents Activity Overview Materials Review of Steps and What is Happening Instruction sheet For Teachers Next Generation Science Standards, Arizona Standards, /Filter /DCTDecode While the banana solution was being mixed, a number two cone coffee filter was laced inside a second five-ounce cup. Extracting quality DNA from scale insects, including mealybugs, can be difficult due to their small body size and waxy coating. Presentation for the Living Faith Series, May 16, 2021 1 0 obj Extraction solution S was added into ziplock Extraction 1 and extraxtion solution SS into ziplock bad Extraction 2. 48 Vitosha Boulevard, ground floor, 1000, Sofia, Bulgaria Bulgarian reg. However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. This could be a problem if two bacteria looked similar because they could easily. 2 0 obj This cookie is set by GDPR Cookie Consent plugin. DNA is made up of a series of monomers called nucleotides. <> In eukaryotic organisms, RNA most ofther carries the genetic information out of the nucleus, where chromosomes reside into the cytoplasm of the cell. << It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity. Some of the protein is still present. These comprise the genetic alphabet which in various combinations, will specify the components of proteins. Spin the stirrer it in place to start gathering the cloudy stuff. >> Write reflection paper about this question. /ca 1.0 We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. * To properly and successfully extract DNA from banana using cell disruption and separation techniques. All of these instructions fit within tiny packages within our tiny cells, so that is all way too tiny for anyone to ever really see or touch, right? Some of the protein is still present. 0000001162 00000 n 20 milliliters (four teaspoons) of distilled water was then added to the mixture. DNA is organized into genes and stores genetic information. /Length 8 0 R ASU - Ask A Biologist. Each strand of the helix consists of a linear polymer made up of genetic building blocks called nucleotides. 0000040917 00000 n Conclusion Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. Thus, DNA is present in all living organisms. Two of the nitrogenous bases, adenine and guanine, belong to a group of compounds known as purines. The cookie is used to store the user consent for the cookies in the category "Other. Breaking cells open to release the DNA. This website uses cookies to improve your experience while you navigate through the website. Materials for each group of students: We understood how a buffer solutions disrupts the plasma membrane and releasing cellular components into the solution. This essay was written by a fellow student. Salt is used at a high molarity due to the fact that it precipitates all of the proteins out. 1. In this biochemistry science project you will make your own DNA extraction kit from household materials and use it to purify DNA from strawberries. * To investigate the effect of temperature on DNA extraction from bananas. Then the salt and shampoo had to be dissolved into the mixture by stirring slowly by avoiding foaming. 6 : Group Bs Results Tan 1 1. Dietary Reference Intakes for Vitamin C . 0000044903 00000 n Let us help you get a good grade on your paper. Extracting DNA from Bananas In the Lab: Extracting DNA from Bananas, DNA was removed from bananas that had been blended with water in order to examine how DNA is seen from the naked eye. You can use it as an example when writing Some of the protein is still present. 135 32 0. g of salt was added into flask SS. This cookie is set by GDPR Cookie Consent plugin. About 60 percent of our genes have a recognizable counterpart in the banana genome! 0000002648 00000 n DNA molecule exists in cells as a long coiled structure often described as a double helix. Gene: a region of DNA that instructs the cell on how to build protein(s). In . Water is a molecule of two hydrogen atoms and one oxygen atom (H2O)more. The banana mixtures were cooled in the ice chest containing ice for 2 minutes. My The cookie is used to store the user consent for the cookies in the category "Performance". This DNA extraction is simple and works every time but the DNA is not pure. After stirring for five to ten minutes, the mixture of banana and shampoo was filtered by pouring it into the coffee filter and letting the solution drain for several minutes until there was approximately five milliliters of filtrate to test. Learn more about Melissa Wilson Sayres' work with Monster DNA. Then alcohol added to the solution causes the DNA to precipitate out. C q" By clicking Accept All, you consent to the use of ALL the cookies. My averages of all three fruits range from a difference compared to the other fruit of 1.67, 0.33 and 2. It would take about 300,000 DNA molecules side by side to make a bundle as thick as a human hair. How do I choose between my boyfriend and my best friend? //= $post_title * Elof Carlson (The Unfit), Mendels Legacy: The Origin of Classical Genetics, Cold Spring Harbour Laboratory Press, USA * Benjamin Cummings(2005), iGenetics: A Mendelian Approach, Pearson; University of Chicago, USA. During the procedure of the lab, a ratio of one banana per one cup (250 milliliters) of distilled water had to be mixed together in a blender. Materials: Half a peeled banana distilled water plastic sealable bag or beaker plastic cup shampoo or dishwashing liquid table salt dna-extraction-lab-report-banana-conclusion 2/7 Downloaded from kelliemay.com on October 4, 2022 by guest sprint against other world-class researchers to solve one of science's greatest mysteries gives a dazzlingly clear picture of a world of brilliant scientists with great gifts, very human ambitions, and bitter rivalries. The information in DNA tells our bodies how to develop, grow, and work. Genetic Engineering of Plants. Necessary cookies are absolutely essential for the website to function properly. And how did we get inside the cells and get the DNA to stick together? If I mash up a living organism , then I can isolate the DNA . DNA is present in the cells of living oranisms. Lab Report Banana Dna. 135 0 obj <> endobj By volunteering, or simply sending us feedback on the site. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc. 10 minutes with: Explore how the human body functions as one unit in harmony in order to life //= $post_title A. potential of crossing over in meiosis I B. 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We even have genes in common with the banana plant! The conical flasks was labelled S and SS. There are four types of nucleotides which vary depending on the four nitrogenous bases of the molecule. Studying DNA is one way scientists learn about what is necessary for life. Banana and Strawberry image by Ralph Daily via Wikimedia Commons. 0000003225 00000 n * To observe the extraction of genomic DNA from plant cells. 20 mL DNA extraction buffer (15 g NaCl/ 1 L water) 1 mL liquid dishwashing detergent Ice -cold 95% ethanol or 95% isopropyl alcohol 1/3 banana . REFERENCES: BOOKS: * Neil A. Campbell, Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson, Biology (8th Ed), Pearson International Edition: Pearson, Benjamin Cummings. xbbbd`b``3] The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. In order to extract DNA you must macerate a thumb sized piece of Banana in a zip lock bag.Then weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue maceratingMeasure out 9.0 mL of water and add to the bag.Then continue macerating.See Ms.Leavitt to get 1mL of detergent soap then continue macerating.Once a thick pace called /Width 625 "Seeing DNA". Segments, or pieces, of DNA are called "genes." Hear Let this two-layer mixture sit for eight minutes. Community Solutions, The Mysterious Case of the Missing Periods, http://owl.english.purdue.edu/owl/resource/560/10/, http://owl.english.purdue.edu/owl/resource/717/04/, http://owl.english.purdue.edu/owl/resource/747/08/, 1/2 peeled ripe banana (you can also use strawberries or other fruit), very cold rubbing alcohol (isopropyl alcohol) placed in freezer ahead of time. Extract: to draw or pull something out (especially from another material). D. student in bioinformatics at the University of Georgia. Once extracted, DNA could be observed and inferences made about the nature of DNA, and then compare the differences between two DNA's amount. The remaining two bases, cytosine and thymine, are known as pyrimidines. They produce molecules called proteins that do most of the work in the body. Need urgent help with your paper? The filtrate, or filtered solution from the bag, was then placed into a test tube. DNA contains the instructions required to build each living thing. 4.1. the solution. the DN +co$or' texture', The soap so$ut#o" co"ta#"s so%#um $aure$ su$!ate' wh#ch, the soap pu$$s apart the !ats +$#p#%s a"% prote#"s that. Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . When subjected to certain conditions, it is possible to collect large amounts of DNA to make it visible. In the cytoplasm, the information in RNA is translated into proteins, which serve as the end products of most all genes. Why or why not? Scholars Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors. 0000008293 00000 n 5 0 obj The salt contributes positively charged atoms that neutralise the normal negative charge of DNA. <>>> The Mendelian Revolution: The Emergence of Hereditarian Concepts in Modern Science and Society. The soap breaks apart the cell membrane and the cell parts that hold the DNA. 0000040445 00000 n OBJECTIVES * To learn basic DNA extraction processes. After the alcohol layer is set up, wait for eight minutes. It does not store any personal data. /Producer ( Q t 5 . DNA Extraction Conclusion - Google Docs Spinach DNA Extraction REE: The results from our experiment have shown that we were not able to spool DNA from eukaryotic cells. DNA or deoxyribonucleic acid is found in all living things. 3) /Subtype /Image As part of the chromosomes, the information contained in genes can be transmitted faithfully by parents through gametes to their offspring. Within every living organism, most cells contain a complete set of DNA instructions. Complementarity serves as the basis for both the replication of DNA and for the transcription of DNA into RNA. Add the dishwashing soap into the bag and gentlymix, trying not to make it foam up. What is the purpose of extracting DNA from banana? every $#v#"g orga"#sm. Essay. ?>, Order original essay sample specially for your assignment needs, https://phdessay.com/extracting-dna-from-bananas/, A Passion for DNA: Genes, Genomes, and Society, A role for transportin in the nuclear import of Adenovirus core proteins and dna, get custom /Creator ( w k h t m l t o p d f 0 . Lea being the main narrator, sat up on her knees, Inviting the audience In to listen and seemed more comforting and engaged, whereas Phil communicated with the audience with the. These cookies ensure basic functionalities and security features of the website, anonymously. Press ESC to cancel. (2018, Feb 11). PhDessay is an educational resource where over 1,000,000 free essays are 19 April, 2016. https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. The authors pay attention to genetic basis. 20g of banana was added into each ziplock bag labeled Extraction 1 and Extraction 2 3. Throughout the entire experiment there were many possible ways that the ending result of the appearance of the DNA could have differed. 4 0 obj . /Type /Catalog 0000002685 00000 n Step 8: Pour the drained liquid into a glass. Mush the banana in the resealable bag for about a minute until all the lumps are gone and it almost looks like pudding. /SA true 2 0 obj The enzymes in softens the phospholipids in the cell membrane and denatures the DNAse Test 2 Amount of DNA extracted Figure 1. In this activity each material plays a specific role in helping to extract the DNA from the cells. Why do you put liquid soap in a banana strainer? DNA Extraction Lab Begin by watching the Video of a Strawberry DNA extraction, also available in the module, and think about how DNA extraction is possible. (c) Or, slowly pour the alcohol down the inside of the test tube with a pasteur pipette or medicine dropper. Each living organism has its own unique DNA sequence. Essentials of Genetics. endobj Three examples of why scientists might need to indentify DNA are for genetic testing, body identification, and analysis of forensic evidence. DNA provides a blueprint for an organisms growth and development. This process was repeated for 4 times. But opting out of some of these cookies may affect your browsing experience. MATERIALS AND APPARATUS PER CLASS * (60-70 oC) Water bath * 95% Ethanol * Extraction solution * Cheesecloth Ice chest containing ice PER GROUP * 40g Banana * 2 ziplock bags (Label Extraction 1 and Extraction 2) * 2 funnels * 2 test tubes * 50ml conical tube (2 pieces) * 500ml beaker (2 pieces) * Glass Rod (2 pieces) * Shampoo 4ml * Distilled water 40ml * Table Salt (NaOH) 0. All the methods employ agarose gel electrophoresis to separate the DNA molecules. Motivation: Recent technological promotions in the field of genomics have resulted in Following Generation DNA Sequencing Technologies. If you are interested in helping with the website we have a Volunteers page to get the process started. Retrieved March 2, 2023 from https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. %PDF-1.4 % Body identification is a subfield of forensic science wherein investigators need to identify a body. Then the DNA can be precipitated out of the solution by adding alcohol. The liquid soap is added to help break down cell membranes to release the DNA. Equipment You will need the following: Two clear glasses/cups Sealable plastic bag Banana Blunt knife and teaspoon Plate/chopping board Measuring jug The bananas were mashed for about 5 minutes. Sometimes it is hard to do all the work on your own. We manage to learn basic DNA extraction processes. The test tubes were carefully handled to avoid shaking. 2 Mar 2023. https://askabiologist.asu.edu/activities/banana-dna. Question 16 options: All of the above Improve crop production Improve disease resistance Generate new, 1 ________ is seen when ABCDEFGHI becomes ABCFEDGHI. you to an academic expert within 3 minutes. 9 How does detergent help to break down DNA? Approximately 3ml of banana solution were dispenced into each test tube. What is the purpose of extracting DNA from banana? The sa$t e"ab$es the DN stra"%s to, The DN prec#p#tates out o! For instance, the detergent or soap helps to break down the cells outer membrane, and the salt helps to separate the DNA from other materials in the cell. Order custom essay Extracting Dna from Bananas For more info, see, Modern Language Association, 7th Ed. 1 0 obj endobj The a$coho$ separates the DN !rom the other, ce$$ compo"e"ts' wh#ch are $e!t beh#"% #" the water, Do not sell or share my personal information, https://www.youtube.com/watch?v=ew9-YGrgpWo. 6T3G]Zq`S;z*H27$21a4a@ aaSn;,8@ U Genetic testing can best be defined as a process in which an individuals DNA is isolated and tested for the presence of specific genes or defects that could indicate the future onset of some disease. The banana DNA was circular and was very separated into many smaller pieces. These cookies will be stored in your browser only with your consent. This DNA extraction is simple and works every time but the DNA is not pure. 0000008815 00000 n The four nitrogenous are A(adenine), G(guanine), T(thymine) and C(cytosine). Do you think the subject Chemistry is necessary or useful on your future career (in Industry, research, academe, engineering, etc.)? DNA stands for deoxyribonucleic acid, which is a nucleic acid that contains the sugar deoxyribose. In a separate five-ounce cup, a solution consisting of one teaspoon of shampoo and two pinches of salt was made. Step 2: In a separate bowl, mix the washing up liquid, salt and tap water. And because the DNA doesnt dissolve in alcohol, this substance helps the DNA clump together in a separate layer. This procedure is designed to extract DNA from kiwi in sufficient quantity to be seen and spooled. This is the DNA pieces clumping together. Aim: To extract DNA from a banana using simple household items Hypothesis: DNA is found in the cells of every living organism. 0000000016 00000 n Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. Did you know that we have over 70,000 essays on 3,000 topics in our We are going to use fruits because they are soft and easy to pulverize. But with bananas, we share about 50 percent of our genes, which turns out to be only about 1 percent of our DNA, emails Mike Francis, a Ph. HSj@WcczG]A)-84H$$`;:4yofRSLGiqU"M l 8cjzR. "Seeing DNA". Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. w !1AQaq"2B #3Rbr The test tubes were then observed. ?>. 6. What does soap do when you wash your hands. Analytical cookies are used to understand how visitors interact with the website. Which DNA extraction the best? Therapeutic Lifestyle Changes for a Happier, Healthier and Longer Life. Then eight millilitres of DNA buffer was then added to the pure. 3 0 obj Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. Such mutants from allelomorphs. %&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz Do this for 30 to 45 seconds. The filtration step (pouring the mixture through the strainer) allows for the collection of the DNA and other cellular substances. Altering Animals. Step 1: Chop up the banana. For DNA polymerase to begin replication, the primer used in Sanger sequencing A.can have any nucleotide sequence B.will have a complementary sequence to the 3 end C.will have a. can use them for free to gain inspiration and new creative ideas for their writing DNA is organized into genes and stores genetic information. During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution. << support@phdessay.com. << The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs. (60 oC) 2. Hypothesis: Order custom essay Strawberry DNA Extraction Lab Formal Write Up with free plagiarism report GET ORIGINAL PAPER /Height 155 6 (5 points) Which of the applications of genomics listed below can be used in agriculture? /Title () Place the coffee filter in a clear glass cup, securing the top of the filter around the lip of the cup. DNA Extraction from Kiwifruit Student Instructions Introduction DNA is present in the cells of all living organisms. 10 What do we have in common with the banana plant? How does detergent help to break down DNA? endobj The positively charged sodium ions in the salt help protect the negatively charged phosphate groups that run along the backbone of the DNA. DNA I have also made the conclusion that filter paper is a neccissity. DNA is a component of living and once-living things. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". Furthermore, analysis of forensic evidence is defined as the application of forensic science and technology to identify specific objects from the trace evidence they leave, often at a crime scene or the scene of an accident. 0000040688 00000 n This cookie is set by GDPR Cookie Consent plugin. o/ H|9 O7ze~.p -s'NK SNlg7WO dq=}i43 So when seeing it without a high-poweredmicroscopewhat does DNA look like? The point both 3 small test tubes and to investigate the cell nucleus to release the DNA. You also have the option to opt-out of these cookies. When the size of the sample nearly tripled at 939.46 ng/ul for the RNA reading, the measurements at both the 260 and 280 wavelength . The probable reason for the tinted brown shade in the human epithelial DNA and not the strawberry DNA is that human epithelial DNA is extracted from one's mouth in which. My hypothesis was correct for strawberries producing the most DNA followed by kiwis and than bananas last. All of the above steps were completed in the same manner for the banana DNA extraction except DNA and small cell debris to filter through. DNA is a component of living and once-living things. Bananas and strawberries are great choices. Why is working with linked genes not simple? Of those 60 percent, the proteins encoded by them are roughly 40 percent identical when we compare the amino acid sequence of the human protein to its equivalent in the banana, Brody adds. % Once these membranes are broken apart, the DNA is released from the cell. The white DNA could be seen precipitating out of the alcohol layer. The salt will help the DNA stick together once it is out of the cells. DNA provides a blueprint for an organism's growth and development. endobj The procedure contains three steps (digestion, Incubation, and centrifugation). Views 778 Extracting DNA from Bananas In the Lab: Extracting DNA from Bananas, DNA was removed from bananas that had been blended with water in order to examine how DNA is seen from the naked eye. * To understand how a buffer solutions disrupts the plasma membrane and releasing cellular components into the solution. This cookie is set by GDPR Cookie Consent plugin. /Type /ExtGState As a human, you usually get a set of instructions from your mom and another set from your dad more, Molecule: a chemical structure that has two or more atoms held together by a chemical bond. 0 What do we have in common with the banana plant? What is the best fruit to extract DNA from? Johns Hopkins University Press. The total number of DNA for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6. Explain what procedures are required to release DNA from a plant cell . 19 Apr 2016. database? 8. /Type /XObject and animals have DNA (deoxyribonucleic acid). ASU - Ask A Biologist, Web. harmony in order to life, Extracting Dna from Bananas. Scientists, teachers, writers, illustrators, and translators are all important to the program. The cookies is used to store the user consent for the cookies in the category "Necessary". Gentlymush the water and banana for 45 seconds. Experiment to purify DNA from fruit Bananas, kiwis and strawberries all work well. A test tube of cold alcohol was then obtained. 0000001613 00000 n All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. You are looking at DNA! /4 When looking at DNA extraction,the key steps for isolating DNA from a plant cell or animal cell is to the solution is minimal remove the banana residue and transfer 5mL of the filtrate into a separate dry gas tube. Three heaping teaspoons of the banana mixture that was made in the beginning of the experiment was then added to the shampoo, salt, and water solution and mixed with a spoon for five to ten minutes. If the lab is conducted properly then we should be able to view a visible. 0000001339 00000 n Banana bunchy top of your problem is a few minutes without seeds and reseeding of cookies before placing the parts of genomic distribution, but any spills. Begin typing your search term above and press enter to search. Studying DNA is one way scientists learn about what is necessary for life. Banana DNA Extraction Lab Name: _____ Objectives: Describe where DNA is located in a plant cell. /CA 1.0 0000044671 00000 n In this activity, you will extract and observe DNA from bananas. Possible Materials and Equipment Measuring cup . <<37B5D6E5CA566B46AF6D8E49138E4E7A>]>> Explore how the human body functions as one unit in 0tx AA~/''8szs' / J~tG x #. <> /SMask /None>> The salt enables the DNA strands to come together, or aggregate. 0000045399 00000 n What is DNA extraction and what is its purpose? re$ease% !rom the ce$$. Variants of genes, called alleles, are responsible for differences in hair color, eye color, and earlobe shape. Because DNA exists inside of cell and ultimately inside of the nucleus proteins must be denatured to locate the DNA. The DNA kiwi extraction shows how simple it may be to extract DNA from a living organism which is very essential because DNA gives important information DNA.